Researchers at University of California, Los Angeles developed a new device called “biophotonic laser-assisted surgery tool, or BLAST” may eventually help scientists study the development of disease, enable them to capture improved images of the inside of cells and lead to other improvements in medical and biological research.
The highly efficient automated tool delivers nanoparticles, enzymes, antibodies, bacteria and other “large-sized” cargo into mammalian cells at the rate of 100,000 cells per minute significantly faster than current technology, which works at about one cell per minute.
Courtesy of Eric Pei-Yu Chiou
Currently, the only way to deliver so-called large cargo, particles up to 1 micrometer in size, into cells is by using micropipettes, syringe-like tools common in laboratories, which is much slower than the new method. Other approaches for injecting materials into cells — such as using viruses as delivery vehicles or chemical methods — are only useful for small molecules, which are typically several nanometers in length. (A nanometer is one one-thousandth of a micrometer.)
The new device “BLAST”, is a silicon chip with an array of micrometer-wide holes, each surrounded by an asymmetric, semicircular coating of titanium. Underneath the holes is a well of liquid that includes the particles to be delivered.
Researchers use a laser pulse to heat the titanium coating, which instantly boils the water layer adjacent to parts of the cell. That creates a bubble that explodes near the cell membrane, resulting in a large fissure — a reaction that takes only about one millionth of a second. The fissure allows the particle-filled liquid underneath the cells to be jammed into them before the membrane reseals. A laser can scan the entire silicon chip in about 10 seconds.
Inserting large cargo into cells could lead to scientific research that was previously not possible. For example, the ability to deliver mitochondria, could alter cells’ metabolism and help researchers study diseases caused by mutant mitochondrial DNA. It could also help scientists dissect the function of genes involved in the lifecycle of pathogens that invade the cell and understand the cell’s defense mechanisms against them.
The research was published online in Nature Methods on April 6.
